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KMID : 1041219940360020157
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Journal of Sericultural and Entomological Science
1994 Volume.36 No. 2 p.157 ~ p.161
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Expression and Morphology of Crystal Proteins in Bacillus thuringiensis subsp. kurstaki Cry-B
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Park, Hyun Woo
Kim, Ho San/Kim, Young Hoon/Jin, Byung Rae/Kang, Seok Kwon
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Abstract
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To investigate the morphology of Bacillus thuringiensis crystal proteins, two type crystal protein genes, crylA(c) gene under the control of crylA(b) gene promoter and crylA gene under the control of its own promoter, were transformed in B. thuringiensis acrystalliferous mutant strain and the transformants were characterized by SDS-PAGE and scanning electron microscopy. The expression and formation of crystal proteins in B. thuringiensis subsp. kurstaki Cry B revealed that crystal proteins appear to have same molecular weight and morphology to those of wild type strain´s, suggesting that the expression and formation of crystal proteins affected not by host cell or recombination of crylA(c) gene under the control of cryA(b) gene promoter but by only structural fragment of protoxin.
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